FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10603227
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1123031.0591398260171172TruSeq Adapter, Index 6 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166890.1573954796968885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC133150.061.4349144
TATGCCG133550.061.25263646
GTCACGC139900.059.0077229
GTATGCC139550.058.6682345
ATGCCGT139900.058.2756647
TCGTATG142200.057.83933643
CACGCCA145100.056.72688731
CGCCAAT145800.056.33676533
TCACGCC145900.056.2973530
TATCTCG144750.056.2695739
ACGTCTG167250.056.18716415
AGTCACG148550.055.8266528
CTCGTAT145850.055.65779542
GCACACG169400.055.6346111
GCCAATA149250.054.96516434
CACACGT172300.054.73825512
ACACGTC173750.054.40159613
GCCGTCT150900.054.37081549
TGCCGTC150950.054.2613848
ACGCCAA153300.053.64781632
Produced by FastQC (version 0.11.2)