FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10228586
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG948570.9273715839119894TruSeq Adapter, Index 5 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG187530.1833391242934263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC109500.061.5964544
TATGCCG109250.061.3915346
GTATGCC113100.059.57539445
ATGCCGT114650.058.5303847
TCGTATG115900.058.13527343
ACGTCTG141500.057.12499215
GCACACG141900.057.00868611
CTCGTAT116500.056.5898742
CACACGT143500.056.42167712
CACGTCT149600.054.1687714
CACAGTG124400.054.15956533
GCCGTCT124200.053.86318249
CACACAG128400.052.68631
ACACGTC154750.052.49930613
AGCACAC155700.052.1550410
TCTCGTA126500.052.0345541
ACACAGT132800.050.86345332
TCCAGTC158500.050.66905225
TGCCGTC132500.050.59326648
ACTCCAG160800.050.1373923
Produced by FastQC (version 0.11.2)