FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11408215
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1017500.8919011431674456TruSeq Adapter, Index 4 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186970.16389067001279342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG121600.061.45606646
CGTATGC122650.061.06952344
GTATGCC125200.059.7435545
ATGCCGT127900.058.69943647
TCGTATG129250.058.03141443
ACTGACC128950.057.8652732
GCACACG150250.056.84749611
CACACGT150850.056.71285612
ACGTCTG150500.056.7104515
CTCGTAT128600.056.09650442
CACGTCT155850.054.78487814
CACTGAC137850.054.2794631
GCCGTCT138200.054.1743249
ACACGTC158200.053.9914513
AGCACAC163200.052.547310
TCTCGTA140900.050.97779541
AGTCACT150400.050.5295728
TGCCGTC149900.049.9928148
TCCAGTC169550.049.9299825
CAGTCAC153800.049.7720627
Produced by FastQC (version 0.11.2)