FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11556103
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1033700.8945056997155528TruSeq Adapter, Index 3 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150880.13056304534495755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG120300.060.5137646
CGTATGC121000.060.41846544
GTATGCC123950.058.8980345
TAGGCAT126150.057.51035735
ATGCCGT127000.057.37563347
TCGTATG128100.057.1223843
TTAGGCA130900.055.9237234
CACTTAG132400.055.8889431
ACGTCTG148700.055.10033415
CTCGTAT130750.054.7489942
GCACACG149700.054.6835711
ACTTAGG135450.054.3248332
CACACGT152450.053.7200112
GCCGTCT135850.053.48734749
TGCCGTC138500.052.58850548
ACACGTC156750.052.2470313
CACGTCT159550.051.33123814
TCTCGTA139400.051.2288741
CATCTCG139200.051.07853739
TCACTTA147050.050.41478730
Produced by FastQC (version 0.11.2)