FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12073361
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1025020.8489930848584747TruSeq Adapter, Index 2 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG211400.1750962304531439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC119750.060.8753744
TATGCCG121800.059.740246
GTATGCC127400.057.19526745
ATGCCGT127650.057.16504347
TCGTATG130150.056.1434943
GCACACG161950.055.85084511
ACGTCTG162850.055.62922315
CACACGT163900.055.1865912
ACACGTC168800.053.66702313
TATCTCG134200.053.650239
CTCGTAT135050.053.49289342
CCGATGT137800.053.098733
CACGTCT170800.053.0794414
CGATGTA139800.052.388234
TGCCGTC141650.051.5409848
GCCGTCT143050.051.18021449
AGCACAC179650.050.5975710
TCTCGTA144950.049.60073541
CGTCTGA184050.049.29791316
TCCAGTC183900.048.60367625
Produced by FastQC (version 0.11.2)