FastQCFastQC Report
Wed 5 Apr 2017
HJWYGBGX2_n01_jes_013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWYGBGX2_n01_jes_013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12720051
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1194650.9391864859661333TruSeq Adapter, Index 1 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG226250.17786878370220371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG142800.061.65724646
CGTATGC143750.061.20032544
GTATGCC147450.059.66554645
TCGTATG151050.058.31067743
ACGTCTG193100.058.06493815
ATGCCGT152100.058.0474347
GCACACG194950.057.52823311
CACACGT196250.057.0772912
CTCGTAT151750.056.3352742
ACACGTC203300.055.2683113
CACGTCT202650.055.2596314
GCCGTCT163150.053.947649
AGCACAC212300.052.93991510
GTCACAT171100.051.8972229
TGCCGTC171450.051.4770348
TCCAGTC214800.051.31278625
CTGAACT219200.051.23048419
TCTCGTA166350.051.2241941
CAGTCAC179050.050.8260127
ACTCCAG219950.050.61381523
Produced by FastQC (version 0.11.2)