FastQCFastQC Report
Thu 21 Oct 2021
HJWL3DRXY_n01_S2Tak.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWL3DRXY_n01_S2Tak.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15472693
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTATACCATCTCGTA1205090.7788495512707452TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTATACCATCGCGTA414020.2675810862401264TruSeq Adapter, Index 22 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT401570.25953465243574597Clontech SMART CDS Primer II A (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTATACCATCTCGGA364980.23588653894961917TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTATACCATCTCGTT350210.22634068936803697TruSeq Adapter, Index 22 (97% over 37bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT187890.12143328895622758Clontech SMART CDS Primer II A (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTATACCATCGCGGA158750.10260010975464968TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)