FastQCFastQC Report
Thu 21 Oct 2021
HJWL3DRXY_n01_S1Tak.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWL3DRXY_n01_S1Tak.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254314530
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGAAATCTCGTA56180382.209090451890421TruSeq Adapter, Index 20 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGAAATCGCGTA17627500.6931377456097376TruSeq Adapter, Index 20 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6372340.2505692458861867Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3372090.1325952551747633Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)