FastQCFastQC Report
Thu 21 Oct 2021
HJWL3DRXY_n01_S1Nug.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWL3DRXY_n01_S1Nug.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14224047
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTCCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCC358210.2518340947551706No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACCTAAATCTCGTA227580.15999665917864303TruSeq Adapter, Index 4 (97% over 38bp)
CTTTGTGTTTGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTT220500.15501917281347566No Hit
GTGTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT168870.11872148622681014No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150530.10582782804359407No Hit
GTGTTTGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCA143510.10089252376626708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)