FastQCFastQC Report
Thu 21 Oct 2021
HJWL3DRXY_n01_G2M2Tak.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWL3DRXY_n01_G2M2Tak.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79594523
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAACAGCATCTCGTA9320711.1710240414406403TruSeq Adapter, Index 5 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3227410.40548141735832754Clontech SMART CDS Primer II A (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAACAGCATCGCGTA2921100.3669976136423357TruSeq Adapter, Index 5 (97% over 36bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1756380.22066593702684792Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)