FastQCFastQC Report
Thu 21 Oct 2021
HJWL3DRXY_n01_G11Nug.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWL3DRXY_n01_G11Nug.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14508436
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTCCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCC473760.32654105514888027No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAAGACCATCTCGTA223270.1538897783331022TruSeq Adapter, Index 2 (97% over 36bp)
CTTTGTGTTTGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTT195510.1347560825991168No Hit
CTTTGTGTTTGATTCCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGA163430.11264480885465533No Hit
GTGTTTGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCA156870.10812330150541381No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156030.10754432800337681No Hit
GTGTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT145530.10030715922791401No Hit
TGTTTGATTCCCCTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCC145410.10022444872762303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)