FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_ydcC_5_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_ydcC_5_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11706053
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA207960.17765168157020986No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA177900.1519726589312384No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC144470.12341478378749865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCTA50700.043.17339322
GGCCTAA51900.041.90542223
CTCAGAT55300.041.5306471
GCCTAAC55700.038.98364624
ACACATG62000.035.06704329
CAGGCCT63350.034.60718521
TGAACGC65850.034.2857868
CAGATTG68100.033.8178753
TCAGATT71600.032.1169782
TTGAACG70950.031.8209957
AGATTGA74900.031.7787864
CGGCAGG75800.029.33646618
ACGGTAA59500.028.46252444
AACACAT76900.028.32313228
TCGAACG64700.027.42010540
TGGTAAC18150.026.79635845
CCAATAG12800.026.51773838
ACGCTGG93100.026.4700911
GATTGAA95150.026.3398065
TAACACA83700.026.10665527
Produced by FastQC (version 0.11.5)