FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_ydcC_5_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_ydcC_5_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8719002
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA273700.3139120738818503No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC239770.27499706961874765No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT235960.27062730344596775No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT200920.23043921769945688No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA175760.20158270407553527No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA175070.2007913290993625No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG171590.19680004661083916No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA163610.18764762297336324No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA160470.18404629337164966No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA159580.18302553434441235No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT153780.17637339686354012No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA153650.17622429723034816No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA152130.1744809784422575No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT146900.16848258550691925No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA141730.16255300778690038No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC125660.144121999283863No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG122260.1402224704157655No Hit
GATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCA118450.1358527042429856No Hit
CCGAGAGCTAGTCTGTTCATCTCTTCTTACGTTCTCAGACGGGAACGCAA113600.13029014100467004No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG113050.1296593348642425No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA112980.1295790504463699No Hit
CGGGGAGGTAGCGGTGCCCTGTACCTGCAATCCGCTCTAGCAGGGCCGAA105560.12106890215187471No Hit
GGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCG105410.12089686411357631No Hit
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG103330.11851126998250487No Hit
TGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGC102770.11786899463952412No Hit
CACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAG101830.11679088959952066No Hit
CTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAG99550.1141759114173847No Hit
GTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAA90370.10364718347352139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTC42850.041.575612
CTCTAGT48850.035.9012536
TCTAGTT54250.032.4565437
GTGGAGA29450.032.214241
TTGTACT57750.030.855291
CTAGTTG58600.029.748548
ACTCTAG62800.028.3164025
CACCTAG19800.027.756745
CCAATAG23800.026.029632
ACCTCTA66550.025.83520917
GGTAGCG37300.025.5266637
TACGGAT54600.025.5184521
TAGTTGT70150.025.449199
AGGTAGC38700.025.2365326
ACCTACT52700.025.10702333
ACCTAGT21950.024.7191546
GAGGTAG39900.024.6528645
GGGAGGT38950.024.620953
CCTAGTT22800.024.5651347
TACCTCT69900.024.49600616
Produced by FastQC (version 0.11.5)