FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_ydcC_4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_ydcC_4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12695846
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT243420.1917320043107013No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA236630.18638379829118912No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC214980.16933097644694178No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG195230.15377470709710878No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA162910.12831756150791368No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA144680.11395853415361212No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA140330.11053221660061094No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG138200.10885450248845174No Hit
CTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAG137140.10801958372841007No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA134890.10624735051134049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTAG22650.019.9463418
GGGATAG23400.017.9532381
CCAATAG22650.017.6118638
TAGAGGT28600.017.5024749
GGGGGAT34550.017.3270821
TGGGAGT102700.017.2487431
AGAGGTA29500.016.96930310
AACTAGA26550.016.88452719
CACCTAG11900.016.1782475
CCTAGTT14100.016.1366527
ACCTAGT12800.016.1345216
GAGTCGA112900.015.6258554
GTGGGAG83800.015.5826821
ACTAGAG29000.015.57988620
GGGAGTC119700.015.43919852
ACCCACG113550.015.22988711
GAGTACA30950.015.05141324
GTCGAAC115700.015.0059116
AGTCGAA118300.014.942475
GGAGTCG122400.014.9263463
Produced by FastQC (version 0.11.5)