FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_ydcC_4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_ydcC_4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8638000
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAC32550.025.3813291
TCGAACA30750.023.6756062
ATGTGCG32400.021.81642739
AATGTGC32950.021.66479138
AGATTCG39750.021.03719151
CTAACAT21000.019.1704311
CTATGCG15800.018.6089868
ATTCACT43850.018.59123661
TGTGCGT37900.018.55834240
GTGCGTT43200.018.3065741
ACAGGCA42200.018.2475976
TTGGTTC36900.018.2120062
CGCTATG22300.017.8973429
AAGATTC46250.017.8539750
TAATGTG33550.017.52160644
ACGCAGC40100.017.3800424
GTAATGT32500.017.33423643
ATGCCCC43350.017.03860712
CCCTCGG28050.016.97784816
AAGGGGC33200.016.97760229
Produced by FastQC (version 0.11.5)