FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_ydcC_3_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_ydcC_3_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11071571
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA201940.18239507293048113No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT185430.1674830067024815No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC174100.15724958996333943No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG135250.12215971879690787No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA132160.11936878695896003No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA116530.10525154921555396No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA113030.10209029956092049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTAG20800.022.89837518
GGTAGCG33900.022.2004477
GGGAGGT37050.021.5379753
CCAATAG23150.021.1616238
TAGAGGT24200.021.118859
AGAGGTA24900.021.08881410
GAGGTAG37100.021.0401135
AGGTAGC39400.019.3675826
CGGGGAG50900.019.3263031
AACTAGA25250.019.140319
GGAGGTA41550.018.9548664
GGGGAGG44150.018.7091982
TCTCGAG39150.018.32007656
ACTAGAG27250.017.99360320
GTACAAT27900.017.95115326
CAATAGA27500.017.68675239
ACAACTA28150.017.54122717
GAGTACA28300.017.4505424
CACCTAG9450.017.4093725
GGGGGAT30700.017.1046091
Produced by FastQC (version 0.11.5)