FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_ydcC_2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_ydcC_2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8927854
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA150110.16813671012093162No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT113560.12719742056713743No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC91390.10236502523450763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAG20200.025.81188238
CAACTAG20050.025.6733318
AACTAGA24150.022.18457819
TAGAGGT25950.022.1220369
AGAGGTA26100.021.59279410
GGTAACA25250.021.35380613
TCGCTTA23450.020.44132252
CAATAGA26500.020.33515539
GGGGGAT24450.020.3321881
ACTAGAG26700.020.33040220
AGGTAAC28350.020.12681612
GTACAAT27750.019.68734626
GAGGTAA29800.018.79476711
CCTACTA14400.018.71462634
ACCTACT13350.018.61577433
TACTATC14750.018.50513536
GAGTACA30650.018.39620424
TAGAATC29100.018.03727342
CTACTAT14800.017.73586835
ACAACTA30050.017.59541917
Produced by FastQC (version 0.11.5)