FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_ydcC_2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_ydcC_2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6631372
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158600.23916619366248795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAG20900.028.6414249
TCTAGTT18400.027.015037
CCTTAGA23100.026.52123810
CTCTAGT18050.026.1809226
TCCACCT23900.024.7523926
TTAGAGT25400.024.12007112
CTTAGAG25300.023.9384811
TGTACTC20100.023.8559782
CTAGTTG21800.022.3202888
CCACCTT27750.022.0754727
ACCTACT15250.021.57644333
TGTTACC21350.021.48487913
GAGTACA28850.021.4847115
ACACGAG27800.021.28909128
CACCTTA29300.020.9079728
GTTACCT22600.020.29778114
CACCTAC16050.020.28365132
ACCTCTA23150.020.11974117
TTACCCT25800.019.66511540
CCTAGAT25350.019.45973656
Produced by FastQC (version 0.11.5)