FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10727854
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2338342.1796903649136166No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN504500.4702711278509197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCC15150.033.139141
CCTGGTG15500.028.31170
TCCTGGT15900.028.2529769
GATAGAT25350.027.98311830
AGTGTAG50050.027.87384825
GGGATAG25500.027.69232628
ATAGATC27400.027.03504831
TGTACTC16800.026.7342472
GTCCTGG17700.026.16147868
TAGATCG50950.026.04019732
GGATAGA27150.025.87323229
GTGTAGA58500.023.96776826
TATCCCC31700.023.5905571
GTAGATC53000.023.00155428
GGGATAA30600.022.84137229
TAAGATC30000.022.80754733
CTCTAGT18400.022.51356
TCTAGTT18900.022.475997
CTAGTTG19450.022.3830858
CACAGGG32800.022.28572523
Produced by FastQC (version 0.11.5)