FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_168_T5_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_168_T5_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8951891
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA187310.2092407067959161No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT146360.16349618197987442No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC143810.16064762182649453No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA126130.14089760476306068No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG118840.13275407397163347No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT104650.11690267452988425No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA102790.11482490124153656No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC99630.11129492081617168No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA94940.10605580429877888No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA89830.10034751316788822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTAG25150.035.6478918
CCAATAG26000.032.43554338
TAGAGGT31050.031.9049159
AACTAGA28350.031.62414619
GTACAAT31200.029.52179126
ACAACTA32350.029.4448617
AGAGGTA33600.029.1728110
GAGTACA31650.028.77137624
CAATAGA31850.026.80714639
CTAGAGT33550.026.72428121
GGTAACA36150.026.63747813
ACTAGAG34800.026.46855520
GAGGTAA37750.026.3371111
AGGTAAC38800.025.4446412
TAACAAC38550.025.0717715
TAGAATC34150.024.99959242
TCGCTTA29600.024.93980252
ATTAGAG40850.024.0786697
GTAACAA40400.023.83633814
CACATCG38100.021.94663248
Produced by FastQC (version 0.11.5)