FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_168_T4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_168_T4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9680287
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA207260.21410522229351256No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT169900.1755113252324027No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC164710.17014991394366716No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG164700.17013958367143456No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA140800.14545023303544616No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA122460.12650451376080069No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA111970.11566805818877064No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT108330.1119078390960929No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA105060.10852984007602252No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT101280.10462499717208797No Hit
GAAAACTGCAACGACAACCGCTTCACATGCATGCCCATTTTGTTCAGGGA99870.10316842878728698No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA97490.10070982399592078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTAG20850.028.72078118
CCAATAG20550.026.73428238
TAGAGGT25650.024.4272339
AGAGGTA26450.023.82185710
AACTAGA25700.023.57323519
GTACAAT26450.022.64211826
CAATAGA24950.022.30000739
ACAACTA27750.022.20968417
GAGTACA27650.021.53346424
ACTAGAG30500.019.75036820
TAACAAC32900.019.3708215
GAGGTAA32650.019.08442511
TAGAATC29850.018.98968142
TCTAGTT18900.018.7052577
GGTAACA34200.018.12125413
AGGTAAC35200.017.90124912
CCTACTA16100.017.82535634
TCGCTTA27200.017.75093852
CTAGAGT34850.017.48619521
TACTATC16050.017.44188536
Produced by FastQC (version 0.11.5)