FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_168_T4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_168_T4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7980793
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG161030.20177192918047118No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA116200.14559956635888188No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA104580.1310396097229937No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA92060.11535194560239816No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT91890.1151389341886201No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT89940.11269556797175419No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT86980.10898666335538336No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC82820.10377414875940272No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA81830.10253367052622465No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA80090.10035343605579045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGA47000.037.46536610
TGTACTC33050.036.217342
CTTAGAG48650.035.83603711
CTCTAGT33350.035.3725786
TCCACCT50200.034.935486
ACCTTAG51150.034.3563549
TCTAGTT35000.034.3057447
TTAGAGT52200.033.33247412
CACCTTA53900.032.7335788
CCACCTT55750.032.0860947
GAGTACA54250.031.88810515
CTAGTTG37450.031.4079198
ACACGAG58800.028.46732328
TTTCCAC63450.028.3017274
TACTCTA43400.027.9876844
ACCTCTA42150.027.25190217
ACTCTAG43500.026.9576935
GTACTCT44900.026.581253
TACCTCT43150.026.53868116
CACGAGA64400.026.14962229