FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_168_T3_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_168_T3_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9584352
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC141780.1479286236565602No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT123520.12887673574593253No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA114790.11976813873280114No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT108160.11285061316612746No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA102380.10681994985159142No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA98340.10260474573554894No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA97610.10184308756606601No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT97010.1012170671527924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTC35600.029.6923622
CTCTAGT36150.028.8551256
TCTAGTT38550.027.5127037
CTAACGT24350.023.577661
CTAGTTG44000.023.3893818
ACCTACT33850.021.30148933
TGTTACC47800.021.2422613
ACCTCTA47200.021.14359717
CACCTAC34500.021.1031832
TACCTCT47700.020.99471716
TCCACCT35400.020.8638366
TACTCTA51650.020.7377454
GTTACTA10500.020.3334083
GTACTCT53500.019.9533463
TTACCTC50250.019.92889815
GTTACCT51350.019.91049614
ACCTTAG38550.019.4318189
TGGACAC48850.019.20952828
ACTCTAG54050.019.169515
CCTTAGA39550.019.029310
Produced by FastQC (version 0.11.5)