FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_168_T2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_168_T2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9815919
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC156490.1594247059292156No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA131920.13439393703228397No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC127890.1302883611814645No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT121800.12408415350615667No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG105910.10789616336483623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAG34700.040.94356538
CAACTAG37750.039.2406118
AACTAGA39000.037.8932919
GGTAACA42100.036.34615713
GTACAAT41250.036.16597426
AGAGGTA44400.035.48044610
GAGGTAA45700.034.318311
TAGAGGT46350.033.9113469
AGGTAAC46150.033.9078112
CAATAGA43250.033.33328239
TCGCTTA35850.033.2786652
ACTAGAG46400.032.45524620
GTAACAA48750.031.1013414
ACAACTA50000.030.53592517
GAGTACA48750.030.53154424
CTAGAGT49650.030.1897121
TAGAATC47250.029.62122542
ATTAGAG55400.028.497337
CACATCG50750.026.33547848
ACATCGC51150.026.06099549
Produced by FastQC (version 0.11.5)