FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n02_168_T2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n02_168_T2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12762980
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC1430561.1208667568232498Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG927430.7266563138075904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550200.43109054468470526No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG493240.3864614690299601No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT202980.15903809298455376No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATA197710.15490896326719936Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG246000.060.9985551
CGGTGGT251350.060.4794452
GGTCGCC253750.060.47413356
GTGGTCG257800.059.87624754
GGTGGTC258650.059.44882653
GTCGCCG265100.058.0954757
TACGTGT273500.057.78976439
ACGTGTA275550.057.53738440
CCGTATC244050.057.5012861
AGATCTC218900.057.4129146
CTCGGTG223700.057.1489150
GTACGTG269150.057.12647238
TCTCGGT224050.057.10581249
GTCAGTA283750.056.78952434
AGTACGT280850.056.32919337
TGGTCGC271200.056.2604955
TAGATCT238800.056.10136845
CAGTACG271100.055.852336
GTATCAT247800.055.58608263
GTGTGTC285150.055.5168330