Basic Statistics
Measure | Value |
---|---|
Filename | HJWCLAFXY_n02_168_T2_rep1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12762980 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC | 143056 | 1.1208667568232498 | Illumina Single End PCR Primer 1 (96% over 33bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 92743 | 0.7266563138075904 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 55020 | 0.43109054468470526 | No Hit |
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG | 49324 | 0.3864614690299601 | No Hit |
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT | 20298 | 0.15903809298455376 | No Hit |
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATA | 19771 | 0.15490896326719936 | Illumina Single End PCR Primer 1 (96% over 33bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGGTGG | 24600 | 0.0 | 60.99855 | 51 |
CGGTGGT | 25135 | 0.0 | 60.47944 | 52 |
GGTCGCC | 25375 | 0.0 | 60.474133 | 56 |
GTGGTCG | 25780 | 0.0 | 59.876247 | 54 |
GGTGGTC | 25865 | 0.0 | 59.448826 | 53 |
GTCGCCG | 26510 | 0.0 | 58.09547 | 57 |
TACGTGT | 27350 | 0.0 | 57.789764 | 39 |
ACGTGTA | 27555 | 0.0 | 57.537384 | 40 |
CCGTATC | 24405 | 0.0 | 57.50128 | 61 |
AGATCTC | 21890 | 0.0 | 57.41291 | 46 |
CTCGGTG | 22370 | 0.0 | 57.14891 | 50 |
GTACGTG | 26915 | 0.0 | 57.126472 | 38 |
TCTCGGT | 22405 | 0.0 | 57.105812 | 49 |
GTCAGTA | 28375 | 0.0 | 56.789524 | 34 |
AGTACGT | 28085 | 0.0 | 56.329193 | 37 |
TGGTCGC | 27120 | 0.0 | 56.26049 | 55 |
TAGATCT | 23880 | 0.0 | 56.101368 | 45 |
CAGTACG | 27110 | 0.0 | 55.8523 | 36 |
GTATCAT | 24780 | 0.0 | 55.586082 | 63 |
GTGTGTC | 28515 | 0.0 | 55.51683 | 30 |