FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_ydcC_5_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_ydcC_5_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11706053
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA223710.1911062593002099No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA189890.16221522318410825No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT182520.15591933506537173TruSeq Adapter, Index 1 (97% over 36bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC149620.1278142171404828No Hit
CTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAG120620.1030407089392129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG34650.043.3404935
AGGCCTA48550.042.3126122
CTCAGAT53050.041.243251
GGCCTAA49550.040.96497723
GATAGAT37050.038.73800337
ACTCCAG40600.038.35856623
CGTATGC39550.038.23329546
GCCTAAC53500.037.87585424
TCGTATG38950.037.83711645
CGATAGA38800.037.80295636
ATAGATC34900.036.31080238
CTCGTAT36750.035.7236944
CAGGCCT60500.034.30191821
GTATGCC44100.034.28785747
ACACATG58700.034.28693429
GTCACAG45000.034.2246529
AGCGATA44900.033.60285634
CAGATTG63950.033.5530363
TCCAGTC45950.033.51349325
TAGATCT38050.032.57071739