FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_ydcC_5_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_ydcC_5_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8719002
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA299740.34377787733045595No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC253730.2910080763830539No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT252530.28963177207666657No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT235800.27044379620511616No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA190350.2183162706006949No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA189490.2173299191811173No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA186470.21386622001004243No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA181240.2078678270747042No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT179150.20547076374107953TruSeq Adapter, Index 1 (97% over 36bp)
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA176560.2025002402797935No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA176510.20244289426702733No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG172420.1977519904227571No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA161360.18506705239888693No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT156990.18005501088312628No Hit
GCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCATCTTCTTGCC134200.1539166982643197No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA130350.14950105528132693No Hit
GATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCA125840.1443284449298211No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA124230.14248190331875138No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG122070.14000455556725414No Hit
CCGAGAGCTAGTCTGTTCATCTCTTCTTACGTTCTCAGACGGGAACGCAA117270.13449933834170472No Hit
CACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAG113800.13051952505573458No Hit
CTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAG110290.12649383495955158No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA107200.12294985137060412No Hit
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG99550.1141759114173847No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT98630.11312074478248772No Hit
TGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGC98290.11273079189567797No Hit
GTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAA97780.11214586256546334No Hit
GGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCG96040.11015022132120167No Hit
ATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCG92070.10559694790757015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG37150.043.8188235
ACTCCAG41950.039.7117923
CTCCAGT44350.037.16992624
CACACGT45050.037.12981812
CGATAGA42200.036.9161736
AGTCACA44600.036.729128
GCACACG47150.035.69875311
GATAGAT41650.035.47022637
CTCGTAT41850.035.05157544
ATAGATC39300.035.00874738
AGCACAC48900.034.85056310
TCGTATG46250.034.5890545
CACGTCT48400.034.5598814
CGTATGC46450.034.43814546
TCCAGTC48850.034.03383625
AGCGATA50150.033.0178934
CCAGTCA50250.033.01673526
GTCACAG50200.032.70174429
CACAGCG50250.032.67145531
AGATCTC41400.032.1354740
Produced by FastQC (version 0.11.5)