FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_ydcC_4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_ydcC_4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12695846
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA269240.21206936505058427No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT262100.20644547830841678No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC227660.17931849519913837No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG194590.15327060520425342No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA175630.13833658662841375No Hit
CTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAG158660.12497000987567114No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA154830.12195327511061492No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG150680.11868448939913102No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA148000.11657356272279926No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA144640.11392702778530868No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA131540.10360869216592576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG27850.017.970323
TGGGAGT91400.017.0812111
ACTCTCG29400.016.90730932
GGGATAG22700.016.6543581
CCAGGGG29150.015.97448842
GAGTCGA100950.015.914464
TCTCGCG31750.015.76733434
CGCGTCT25800.015.6030021
GGGAGTC106800.015.3705912
GGAGTCG109600.015.297273
ACCCACG102350.015.21434911
CGCGCAT32950.014.87489737
GATTCGA72850.014.846174
GTCGAAC105000.014.7339276
CTCTCGC34700.014.72855933
TGCTACG33600.014.58064770
TCGCGCA35700.014.51301836
ACCTAGT10150.014.4833416
GGTACCA26350.014.4787634
AGTCGAA109850.014.3064485
Produced by FastQC (version 0.11.5)