FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_ydcC_4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_ydcC_4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8638000
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC90780.10509377170641351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACT32850.036.44167328
GTCACTC37400.032.47659329
ACTCTCG36650.032.47376332
TCGCGCA36250.032.35151736
TCTCGCG38700.031.20800434
ACTCCAG40000.029.74926223
ACGTCTG41900.029.73225615
CACGTCT44000.028.3927514
GCACACG44650.027.58738711
CTCGCGC45650.025.99646435
CTCGAAC30050.025.3933261
GAACTCC48350.025.18762821
CACTCTC48050.025.06125331
ACACGTC51300.024.48888213
AGCACAC52300.024.02050610
CAGTCAC51450.024.01537327
TCCAGTC50300.024.00703625
GCGCATC41700.023.75790838
CGCGCAT47400.023.55931337
AACTCCA52250.023.37551522
Produced by FastQC (version 0.11.5)