FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_ydcC_3_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_ydcC_3_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11071571
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA230950.20859731649645746No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT199680.18035380886777497No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC182790.16509852124870084No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA144610.13061380358758481No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG143490.1296022036981021No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA128920.11644237299295647No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA127280.11496110172621392No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA121380.10963213802268892No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA113510.10252384237069878No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA112420.10153933890682723No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT111710.10089805683403015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTAG9850.017.4113395
ACCTAGT10500.017.0001686
GGGATAG22900.016.967151
CAACTAG17700.016.80496218
CTCCGCG21550.015.91907433
GGTGGTG16850.015.7827267
GGGGAGG30000.015.7506522
ACTCCGC21500.015.46750432
CCAATAG17900.015.45119738
GGTAGCG29550.015.3940967
GATTCGA67600.015.1703364
CGCGTCT23350.015.1410451
ACTCCAG21350.015.08082223
TGGGAGT76400.014.8447541
AGAGGTA21350.014.75071210
CCTAGTT13550.014.7198677
CGGTGGG42400.014.5300861
TAGAGGT22050.014.4411299
GAGGTAG32750.014.4276235
CTAACGT21500.014.1645291
Produced by FastQC (version 0.11.5)