FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_ydcC_3_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_ydcC_3_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9551760
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC180650.18912744876336926No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT178400.18677186193957973No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA172130.1802076266572862No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT159850.1673513572367815No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA159260.16673367002521003No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG148540.1555106074691994No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA137590.14404675159342362No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA135310.14165975694531688No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT120260.12590349841285794No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA118760.12433310719699826No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA115980.12142264881027161No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA114750.12013492801326667No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA111100.11631364272134141No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG105420.11036709465061936No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA98500.10312235650811996No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA98210.10281874753972044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGC45050.036.13131732
ACTCCAG49500.034.14835723
CACTCCG49100.033.15138231
CACACGT51400.032.81574212
CTCCGCG50750.032.4875833
AGTCACT52700.031.680228
GCACACG53400.031.5206611
GTCACTC54650.030.35829729
TCACTCC55600.029.33949730
CCAGTCA57450.029.18255826
CTCCAGT58550.029.11009224
CCGCGAA60200.028.02814735
CAGTCAC61450.027.51079427
TCCAGTC61800.027.24100325
CACGTCT63700.026.91875514
AGCACAC65200.025.97701610
TCCGCGA63350.025.91626234
ACGTCTG67600.025.41751715
ACACGTC75900.023.7445413
CGCGAAA69300.023.6408636
Produced by FastQC (version 0.11.5)