FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_ydcC_2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_ydcC_2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8927854
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA166090.18603574834445097No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT122240.13691980177991261No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC95340.10678938074032125No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA92150.10321629363562622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG20600.024.4649523
CACACGT23800.021.02578212
GCACACG24950.020.61756911
CCGGCTA27800.018.38306433
AGTCACC27700.018.19598428
GGCTATG28550.018.14616635
CACCTAG6400.016.9536175
TCGTATG27900.016.93720845
CTCCAGT31350.016.18839624
CCAGTCA31700.016.12061326
CCTACTA11800.016.0193934
CTATGAT29400.015.83645537
TCACCGG34000.015.54648530
ACCTAGT7500.015.4003626
TGGGAGT47250.015.33611
GGGATAG19450.015.29838851
CAGTCAC34700.015.23107527
CCTAGTT9450.015.1823957
CTACTAT12650.014.9428235
TCCAGTC35400.014.92997825
Produced by FastQC (version 0.11.5)