FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_ydcC_2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_ydcC_2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6631372
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT215760.3253625343292459TruSeq Adapter, Index 7 (97% over 36bp)
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA73530.11088203165197189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG52850.039.73138823
CACACGT53700.039.55391712
GCACACG55800.038.37859311
AGTCACC55850.037.29148528
TCGTATG55450.036.74234445
CCGGCTA56500.036.3693133
CCAGTCA57800.036.33481626
CGTATGC57550.036.06881346
GGCTATG58250.035.336535
CTCCAGT59950.035.31966824
TCACCGG59200.034.9481630
CTATGAT55050.034.84988837
CAGTCAC61500.034.1485627
CTCGTAT54250.033.81553644
GTATGCC61050.033.7713847
AGCACAC64150.033.6558310
CACGTCT63850.033.4856414
TCCAGTC64150.033.3373425
ACGTCTG66600.032.20830515
ACACGTC67550.031.91026913
Produced by FastQC (version 0.11.5)