FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10727854
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4043053.768740700609833No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198420.18495777440669867No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT182240.16987554081179704Illumina Single End PCR Primer 1 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT114270.10651710957289315TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC53850.054.496259
TTCCCTA57700.050.802038
CACTCTT59600.048.5365522
CCTACAC62350.048.3458511
CGACGCT60200.048.34521517
TACACGA63150.046.47024513
CCCTACA77500.039.07441310
ACACTCT77950.037.269391
CGAGATC42600.035.31068428
TTTCCCT86750.034.1836057
GACGCTC88150.033.37134618
CTCGTAT53650.033.20207244
ACGCTCT90600.032.58530419
CTTTCCC92000.032.175066
CGCTCTT93750.031.52012420
CACACGT140700.031.48289912
ATGCCGT66150.031.40414849
CACGTCT142750.031.15329614
GCACACG141200.031.07647311
ACGTCTG144750.030.74094215
Produced by FastQC (version 0.11.5)