FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_168_T5_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_168_T5_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8951891
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA202880.22663367996772973No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT154590.17268977023960635No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC146790.16397652741750318No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA136260.1522136496076639No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT119060.13299983210251332No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG113950.12729154097162262No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA111440.12448766411476636No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA103340.11543929656873614No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT98590.11013315510655794No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA91810.10255933634580672No Hit
GAAAACTGCAACGACAACCGCTTCACATGCATGCCCATTTTGTTCAGGGA90480.10107361673639681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAAT23050.027.1852431
GTCACTA24350.026.30687129
ACTAATG24650.025.5624132
ACTCCAG28200.022.0906223
CAACTAG17400.019.91081418
CACCTAG7600.019.8034295
AGTCACT33850.019.23386228
TGCGCAT31450.019.14547337
CCCTAGG1651.5980786E-619.08784168
ATGCGCA33200.018.76927636
CCAATAG17650.018.64431238
TACTATC17400.018.50990536
CTCCAGT35050.018.27350824
TCACTAA35450.018.0715630
TAGAGGT21300.017.9070199
CACACGT35150.017.8201512
AACTAGA20300.017.23860719
CCTACTA19000.016.95089134
ACTATCT19000.016.76664437
CTACTAT19500.016.69559335
Produced by FastQC (version 0.11.5)