FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_168_T5_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_168_T5_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8526599
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA193410.22683135444741803No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT170260.19968102170630989No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT161440.18933692085203022TruSeq Adapter, Index 3 (97% over 36bp)
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT146920.17230785686063108No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA140010.16420380505756163No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC132870.15583000912790668No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA122530.14370325143706184No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT112420.1318462378728025No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG102640.12037624849016589No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA101830.11942628004436472No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA100860.11828866351050402No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA98410.115415302162093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA115300.040.58807829
CACTAAT115800.040.3247531
ACTAATG117150.039.9193532
ACTCCAG119150.039.5636223
CACACGT120650.039.5040612
GATCGGA121000.038.5391271
GCACACG123900.038.46783411
AGTCACT121850.038.462628
CTCCAGT123100.038.29613524
TCACTAA123150.038.14632430
TGCGCAT117200.037.12751837
AGCACAC128850.037.0443410
CCAGTCA126300.036.99527426
ATGCGCA123900.036.98393236
CACGTCT130350.036.53751414
AATGCGC127100.036.3830835
CAGTCAC130800.036.04360227
TAATGCG128900.036.01059334
ACGTCTG133500.035.70160715
ACACGTC135400.035.66583313
Produced by FastQC (version 0.11.5)