FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_168_T4_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_168_T4_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9680287
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA232750.2404370862144893No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT178550.18444701071362862No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC169470.17506712352639958No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG161830.16717479554066939No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA150180.15514002838965416No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA130510.13482038290806875No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT125820.12997548523096475No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA113790.11754816773510951No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA112690.11641183778952008No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT111330.11500692076588226No Hit
GAAAACTGCAACGACAACCGCTTCACATGCATGCCCATTTTGTTCAGGGA108510.11209378399628027No Hit
CTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAG104520.10797200537546046No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA98540.10179450258034704No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA97830.10106105325183024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCAG24950.022.86367223
TATCTCG22450.022.30027441
GAAGCTA25900.021.49065836
TCGTATG30150.019.04193545
CAACTAG15700.018.27785318
TACTATC15450.018.12649536
CTATCTC27900.018.06951740
CTACTAT16000.017.50321635
TGCTACG35750.017.325570
TGGGAGT80600.017.1113531
ACTATCT15850.017.00619337
GTCACCT33400.016.97758929
CACACGT34050.016.95742412
GGGATAG17000.016.6785871
CTCGTAT32800.016.54404344
CCAATAG15050.016.51466438
AGGACTA17850.016.47080853
CCTACTA16600.016.44957234
CTAACCT17150.016.33096542
AGTCACC34500.016.13164928
Produced by FastQC (version 0.11.5)