Basic Statistics
Measure | Value |
---|---|
Filename | HJWCLAFXY_n01_168_T4_rep1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 7980793 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT | 20021 | 0.25086479501473097 | TruSeq Adapter, Index 19 (97% over 38bp) |
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA | 12683 | 0.158919044761592 | No Hit |
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA | 11929 | 0.14947136205637712 | No Hit |
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT | 10147 | 0.12714275385917165 | No Hit |
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA | 9605 | 0.12035144878460072 | No Hit |
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT | 9293 | 0.11644206283761525 | No Hit |
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA | 9029 | 0.11313412088247371 | No Hit |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 8929 | 0.11188111256613222 | No Hit |
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA | 8832 | 0.11066569449928096 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GAAGCTA | 4125 | 0.0 | 40.905437 | 36 |
TATCTCG | 3795 | 0.0 | 40.31592 | 41 |
ACTCCAG | 4255 | 0.0 | 40.3025 | 23 |
CACACGT | 4630 | 0.0 | 38.090527 | 12 |
CTATCTC | 4230 | 0.0 | 36.086285 | 40 |
GCACACG | 4985 | 0.0 | 35.448154 | 11 |
TCGTATG | 4675 | 0.0 | 35.41821 | 45 |
GTCACCT | 4855 | 0.0 | 35.040443 | 29 |
CGTATGC | 4895 | 0.0 | 34.61152 | 46 |
AGTCACC | 5025 | 0.0 | 34.06291 | 28 |
CTCGTAT | 4700 | 0.0 | 33.818478 | 44 |
CAGTCAC | 5150 | 0.0 | 33.235096 | 27 |
CTCCAGT | 5300 | 0.0 | 32.358093 | 24 |
CCAGTCA | 5435 | 0.0 | 31.621122 | 26 |
CACGTCT | 5650 | 0.0 | 31.214008 | 14 |
TCCAGTC | 5655 | 0.0 | 30.45227 | 25 |
ACGTCTG | 5760 | 0.0 | 30.43566 | 15 |
AGCACAC | 5905 | 0.0 | 29.984585 | 10 |
GTATGCC | 5670 | 0.0 | 29.695272 | 47 |
AGCTATC | 5330 | 0.0 | 29.62263 | 38 |