FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_168_T4_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_168_T4_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7980793
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT200210.25086479501473097TruSeq Adapter, Index 19 (97% over 38bp)
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA126830.158919044761592No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA119290.14947136205637712No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT101470.12714275385917165No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA96050.12035144878460072No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT92930.11644206283761525No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA90290.11313412088247371No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC89290.11188111256613222No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA88320.11066569449928096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCTA41250.040.90543736
TATCTCG37950.040.3159241
ACTCCAG42550.040.302523
CACACGT46300.038.09052712
CTATCTC42300.036.08628540
GCACACG49850.035.44815411
TCGTATG46750.035.4182145
GTCACCT48550.035.04044329
CGTATGC48950.034.6115246
AGTCACC50250.034.0629128
CTCGTAT47000.033.81847844
CAGTCAC51500.033.23509627
CTCCAGT53000.032.35809324
CCAGTCA54350.031.62112226
CACGTCT56500.031.21400814
TCCAGTC56550.030.4522725
ACGTCTG57600.030.4356615
AGCACAC59050.029.98458510
GTATGCC56700.029.69527247
AGCTATC53300.029.6226338