FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_168_T3_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_168_T3_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11088101
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA305060.27512375653865345No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT232080.2093054527551652No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC210700.19002352161113972No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG207940.18753436679554056No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA198770.179264240107481No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT169240.15263208731594347No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA163180.14716676913386703No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA145980.13165464492071274No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT140110.12636068159913044No Hit
CTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAAACGTAGCATTAG133750.12062480311101063No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA131880.11893831053667352No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA127080.1146093456399793No Hit
GAAAACTGCAACGACAACCGCTTCACATGCATGCCCATTTTGTTCAGGGA121790.10983846557674755No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT120390.10857585081521172TruSeq Adapter, Index 7 (97% over 35bp)
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA119850.10808884226433363No Hit
GATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTCA115750.10439118474840732No Hit
ATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCG113160.10205534743956608No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG112010.10101819959973309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG45300.033.070728
ACTCCAG46250.033.06746723
TATCTCG34350.031.90339541
GTATCTC39400.028.43591340
TCGTATG39800.027.08960745
CCAGTCA56450.026.84776326
ATTCGTA56900.026.33344536
GTCACGA59600.026.01735529
CAGTCAC59300.025.9708427
CTCCAGT59450.025.84422124
CGTATCT46750.025.46306839
CACACGT59900.025.41199712
CACCTAG10500.024.6670235
CCTACTA17950.024.37954934
CCTAGTT11600.023.8310687
GCACACG63800.023.69384411
ACCTAGT11250.023.6447856
TTCGTAT63700.022.75271237
CTACTAT19450.022.67906635
CCAATAG24200.022.56821438
Produced by FastQC (version 0.11.5)