Basic Statistics
Measure | Value |
---|---|
Filename | HJWCLAFXY_n01_168_T3_rep1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9584352 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT | 48003 | 0.5008476316395725 | TruSeq Adapter, Index 7 (97% over 35bp) |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 14699 | 0.15336456757848627 | No Hit |
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT | 13365 | 0.13944604705670244 | No Hit |
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT | 12223 | 0.12753079185739422 | No Hit |
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA | 12122 | 0.1264769908283836 | No Hit |
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA | 11223 | 0.11709711830283362 | No Hit |
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA | 10759 | 0.1122558937735175 | No Hit |
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA | 10656 | 0.11118122539739775 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATCTCG | 7505 | 0.0 | 54.951668 | 41 |
AGTCACG | 10340 | 0.0 | 54.161762 | 28 |
ACTCCAG | 10595 | 0.0 | 53.181072 | 23 |
GTATCTC | 8385 | 0.0 | 50.394302 | 40 |
CACACGT | 11370 | 0.0 | 50.012897 | 12 |
CCAGTCA | 11325 | 0.0 | 49.387646 | 26 |
CTCCAGT | 11510 | 0.0 | 48.98609 | 24 |
CGTATCT | 9150 | 0.0 | 48.744236 | 39 |
GTCACGA | 11570 | 0.0 | 48.616646 | 29 |
GCACACG | 11790 | 0.0 | 48.260696 | 11 |
CAGTCAC | 11780 | 0.0 | 47.866566 | 27 |
TCGTATG | 8060 | 0.0 | 47.858883 | 45 |
ATTCGTA | 11500 | 0.0 | 47.790882 | 36 |
CGTATGC | 8525 | 0.0 | 46.928688 | 46 |
TCCAGTC | 12155 | 0.0 | 46.33125 | 25 |
CTCGTAT | 8570 | 0.0 | 45.587387 | 44 |
CACGTCT | 12615 | 0.0 | 45.38216 | 14 |
ACGTCTG | 12720 | 0.0 | 45.062798 | 15 |
TCGTATC | 11110 | 0.0 | 44.64816 | 38 |
TTCGTAT | 11890 | 0.0 | 44.544674 | 37 |