FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_168_T3_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_168_T3_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9584352
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT480030.5008476316395725TruSeq Adapter, Index 7 (97% over 35bp)
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC146990.15336456757848627No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT133650.13944604705670244No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT122230.12753079185739422No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA121220.1264769908283836No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA112230.11709711830283362No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA107590.1122558937735175No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA106560.11118122539739775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG75050.054.95166841
AGTCACG103400.054.16176228
ACTCCAG105950.053.18107223
GTATCTC83850.050.39430240
CACACGT113700.050.01289712
CCAGTCA113250.049.38764626
CTCCAGT115100.048.9860924
CGTATCT91500.048.74423639
GTCACGA115700.048.61664629
GCACACG117900.048.26069611
CAGTCAC117800.047.86656627
TCGTATG80600.047.85888345
ATTCGTA115000.047.79088236
CGTATGC85250.046.92868846
TCCAGTC121550.046.3312525
CTCGTAT85700.045.58738744
CACGTCT126150.045.3821614
ACGTCTG127200.045.06279815
TCGTATC111100.044.6481638
TTCGTAT118900.044.54467437