FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_168_T2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_168_T2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9815919
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA148920.15171274335087728No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC137340.13991557998797668No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT136640.13920245266897577No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG114260.11640275352720413No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA105770.10775353790103606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAG21650.026.35635238
ACTCCAG34900.023.16531223
CAACTAG25500.022.36987918
AGTCACA36750.022.19223628
AACTAGA27450.021.2906119
GTACAAT29050.020.6037326
CAATAGA29150.019.57672739
AGAGGTA32200.019.45281210
GGTAACA31050.019.15908413
TGGGAGT49600.018.6323151
TAGAGGT33500.018.4890129
CTAACGT16700.018.4463621
AGGTAAC33950.018.0378912
GAGGTAA34550.017.82583411
TCGCTTA25000.017.77932752
CCAGTCA45700.017.61600726
GAGTCGA51500.017.602714
CAGTCAC46400.017.3502527
CTCCAGT46800.017.20107824
CACACGT48100.017.0966212
Produced by FastQC (version 0.11.5)