FastQCFastQC Report
Fri 5 Apr 2019
HJWCLAFXY_n01_168_T2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCLAFXY_n01_168_T2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12762980
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT8709946.824378005763545TruSeq Adapter, Index 13 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTA1344131.0531474624264867TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGTCGTAT759880.5953781953744345TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTAT671390.5260448578623488TruSeq Adapter, Index 13 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT431830.33834574683968793No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT346210.2712611004639982TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG263890.20676205713712628TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTTT259590.20339293801290922TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGGAT211870.16600355089485372TruSeq Adapter, Index 13 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGTCGTA190540.14929115300658624TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTAGTAT185000.144950473948874TruSeq Adapter, Index 13 (97% over 38bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA159580.12503349531222333No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT157150.12312955124900296Illumina Single End PCR Primer 1 (96% over 30bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT142340.11152567817233906No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAA139960.10966090991288868TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1187850.055.98203344
AATCTCG1266700.055.55822441
TCGTATG1304350.055.39276545
GTATGCC1407250.055.38903447
CGTATGC1367650.055.2945346
GCCGTCT1158500.054.9908251
TATGCCG1398850.054.94143748
ATGCCGT1349300.054.52079449
TGCCGTC1308050.054.34456350
AGTCACA1937100.054.2344928
ACTCCAG1935950.054.23193723
CCAGTCA1931500.054.06789426
CTCCAGT1939900.053.9979824
CAGTCAC1939350.053.9679527
GTCACAT1938100.053.95291529
TCCAGTC1940500.053.8948125
CGTCTTC1128750.053.89340253
CCGTCTT1222300.053.85817352
CACATTC1940250.053.8225431
CACACGT1974650.053.65508312
Produced by FastQC (version 0.11.5)