FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8835080
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3992054.518408435464082No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC295190.3341112927104225Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC77700.062.8933817
GGAGTTC82400.062.1236277
ACGTGTG79700.062.10572416
GTGACTG75300.062.0581321
TGACTGG75950.061.631012
GTGTGCT73300.061.0306118
GCACACG53000.060.8281518
AGACGTG83350.060.33626614
TAACTGA88000.060.2494352
CAGACGT82350.060.22036413
TCAGACG82900.060.07651512
GTGCTCT56300.059.49764320
ACTGGAG80150.059.4162144
AGTTCAG85850.059.3284879
GAGTTCA87250.058.944898
ACTGAAG88150.058.197964
CACACGT41550.058.1291519
GACGTGT86950.057.77272415
TGGAGTT90700.057.763966
ACACGTC26650.057.264520
Produced by FastQC (version 0.11.5)