FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_POLa-5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_POLa-5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10801248
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2203872.0403845925952258No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC121400.11239441960780829Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20000.024.85350218
CGGGGTG11950.019.3912261
CGGGGTT16350.018.8970571
GAGCACA41050.018.84605616
CTCGGCG12650.018.80607470
AGAGCAC41500.018.7263115
GAGGGTC20100.018.1663021
CGGGGTA21000.018.0565051
GAGGGTA33250.017.9509121
AGCACAC32750.017.31573917
CGTGTGC27350.017.27880717
CGGGGAG10400.017.217341
GGGGGTA21050.017.1796511
CGGCGTG8800.017.155961
GACTGGA33850.015.8538343
CGCCGTA63100.015.80563547
GTGACTG29850.015.6435941
ACGTGTG30250.015.62247216
AAGGGGG54450.015.48556667
GGGGGTC10700.015.4220931
Produced by FastQC (version 0.11.5)