FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_POLa-05-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_POLa-05-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12157193
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2176021.789903310739576No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC140840.11584911089262136Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG15250.025.7841241
GCACACG21900.025.41412718
CGGGGAC12500.021.6264341
CGGGGAG13550.020.9869791
CGGGGAT19500.020.7046281
GAGCACA44650.019.9138816
CGGGGTA24850.019.7790681
CGGGGTT19150.019.4330651
AGAGCAC46450.018.91641415
GAGGGTC22400.017.8673881
CGGCGTG10850.017.7966061
GAGGGTA39550.017.6648851
AGCACAC35250.017.57738317
CGCCGTA70000.016.9969447
CCGGGTG11050.016.521341
GGGGGTA24250.016.5043071
CGGGGGT13850.016.4766081
CGGGTTA13850.016.2231221
CAGATCG56750.015.8011186
GGTCGCC77850.015.7320444
Produced by FastQC (version 0.11.5)