FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_POLa-04-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_POLa-04-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7169981
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1231291.7172848854132248No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC167230.23323632238356004Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20200.033.96410818
AGAGCAC38100.030.04412315
GAGCACA38900.028.1662116
AGCACAC28100.026.16017317
CGGGGAC10900.024.1560171
CACACGA15900.023.99619
CGGGGTT12950.023.5852261
CGGGGTG8700.023.4044951
CGGGGAG9350.022.9038641
AAGGGGG43250.022.0042967
ACACGAG17900.021.50982920
CGGGGAT11350.020.7238041
AGGGGGG52050.020.36748368
GGGGGAT12600.020.339621
AAAGGGG46300.020.1013566
CACGAGA19500.019.20642321
GGGGGTA13800.018.8253541
GATCGAG15650.018.7925878
AAGAGCA91650.018.6351614
CACACGT17100.018.42280819
Produced by FastQC (version 0.11.5)