FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_POLa-03-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_POLa-03-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10248494
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1628921.5894237728977547No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC288590.28159259301903283Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27950.033.56541418
AGAGCAC53250.029.45254915
GAGCACA52900.029.38241616
AAGGGGG71350.025.25261167
AGCACAC40050.025.08585717
CGGGGTT16550.024.3972511
AAAGGGG75300.023.7420966
CGGGGTG13700.023.321791
CGGGGAT17100.022.3805851
AGGGGGG82800.022.30963168
CGGGGAC12800.021.1213531
CACACGA21100.021.06938219
GATAGAT78600.020.7590968
AGATAGA79900.020.3361767
CACACGT24700.020.12435319
CGGGGTA21850.020.0862851
GGGGGTA21100.019.801841
GATCGAG24450.019.476248
CCGGGTA15450.019.3166371
CGGGGAG10400.019.2434331
Produced by FastQC (version 0.11.5)