FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_POLa-02-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_POLa-02-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10779679
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1297351.203514501684141No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC134340.12462337700408334Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT20700.027.477521
CGGGGAT19850.024.585961
CGGGGTG15900.024.510871
CGGGGAC15200.023.7917581
CGGGGAG12450.022.842761
GGGGGTA22400.022.1005951
CGGGGTA21900.020.360691
CGGGGCG6100.020.1451761
GCACACG18650.019.89496618
GGGGGAT20650.018.8727781
CGGCGTA10000.018.6083891
GGGGTTA26100.018.4180662
AGAGCAC41950.018.19067415
GAGGGTA36550.017.963341
CGGGGCA12850.017.4867761
CGGGGTC23650.017.2210541
GAGGGTC20450.017.1687851
GGGGTAA28750.017.086542
GGGGGTC12800.017.0064851
GAGCACA42650.016.90722816
Produced by FastQC (version 0.11.5)