FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_POLa-01-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_POLa-01-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14821553
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2591741.7486291753637422No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC819840.5531404165272019Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG47500.039.50089318
GAGCACA86350.035.9181916
AGAGCAC88950.035.57680515
AGCACAC65450.031.07467517
AAGGGGG153100.027.94810967
CACACGA33900.026.95046619
ATAGATC123950.026.7472279
AGGGGGG176050.026.51059368
GATAGAT126650.025.9312118
AAAGGGG162900.025.88016766
CAGATCG137650.025.6472916
AGATAGA129100.025.5776427
CACACGT37500.025.29667719
ACACGAG36800.024.63591220
AAGAGCA191450.024.55178514
ACACTCT60700.023.6546081
GATCGAG38050.022.268248
CGGGGTG21000.022.0666161
CGGGGTT27700.021.418451
CACGTAG34300.021.12452921
Produced by FastQC (version 0.11.5)