FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_POLa-00-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_POLa-00-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9582921
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1925102.0088864345224176No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC129370.1350005911558699Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22100.031.83524318
AGAGCAC45150.025.19748315
GAGCACA44700.024.8245316
CGGGGTG11750.024.8106021
AGCACAC37900.021.05782917
CGGGGTT17050.020.600261
GGGGGTA20950.019.615481
CGGGGTA17300.018.0692861
GAGGGTA31600.017.7840211
CACACGT22550.017.384519
CACACGA18650.017.0786419
GGGGGAC14850.016.5565051
CGGGGAG10200.016.1843321
CGGGGAC12600.016.1679341
CAGATCG57200.016.0444566
CGGAGCG6800.016.012161
AAGAGCA101850.015.97838114
GAGGGTC19150.015.9568641
GGGGGAT16950.015.9557831
GGGGGTC11900.015.9383691
Produced by FastQC (version 0.11.5)